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Stabilization Once the protein has become removed from it is natural environment, it is actually exposed to many agents that can damage that. the proteins can be affected by p H, temperature, proteases, oxidation of disulphide bridges, contamination by simply heavy mining harvests, salt concentration, etc .

These types of variables could be controlled by using buffers, maintain low temperature, by using inhibitors, and so forth Essays Source: protein purification is necessary to detect it is presence to indicate its purity.

Today, due to the molecular cloning tecinicas, new techniques have been produced to obtain protein.

Solubilization Methods The first step intended for the solubilization of a protein is their location in a solution, nevertheless this initial must be introduced from the cellular.

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The idea is always to minimize loosing the desired proteins, but selectively eliminate the various other components of the mixture.

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